Simultaneous Decoupled Detection of Dopamine and Oxygen using Pyrolyzed Carbon Microarrays and FSCV

摘要

Microfabricated structures utilizing pyrolyzed photoresist have been shown to be a useful for monitoring electrochemical processes. These previous studies, however, were limited to constant potential measurements and slow scan voltammetry. Work described in this report utilizes microfabrication processes to produce devices that enable multiple fast-scan cyclic voltammetry (FSCV) waveforms to be applied to different electrodes on a single substrate. This enabled the simultaneous, decoupled, detection of dopamine and oxygen. This paper describes the fabrication process of these arrays and shows that pyrolyzed photoresist electrodes possess comparable surface chemistry and electrochemical properties to PAN type, T-650, carbon fiber microelectrodes using background-subtracted FSCV. The functionality of the array is discussed in terms of the degree of crosstalk in response to flow injections of physiologically relevant concentrations of dopamine and oxygen. Finally, other applications of pyrolyzed photoresist microelectrode arrays are shown, including: spatially resolved detection of analytes and combining FSCV with amperometry for the detection of dopamine.