Development of fluorescent fiber optic single-polymer membrane sensors for simultaneous ratiometric detection of oxygen and carbon dioxide in biological systems

摘要

Oxygen- and carbon dioxide-sensitive fluorescent indicators are incorporated into a polymeric membrane for their detection by ratiometric fluorescence measurements. Oxygen is measured by fluorescent quenching of Ruthenium, and Platinum dyes. Carbon dioxide is monitored by tracking the change in the fluorescence emission spectrum of a pH indicator dye as the gas diffuses through the polymer membrane and interacts with the dye. The detection principle is the pH change induced in the membrane due to the influx of carbon dioxide. The indicator dye studied is HPTS. The polymer membranes are made of silicone or polystyrene.