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Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype

机译：四种单步实时RT-LAMP测定法的开发和验证可特异性检测每种登革热病毒血清型

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Background4 one-step, real-time, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of dengue virus (DENV) serotypes by considering 2,056 full genome DENV sequences. DENV1 and DENV2 RT-LAMP assays were validated with 31 blood and 11 serum samples from Tanzania, Senegal, Sudan and Mauritania. DENV3 and DENV4 RT-LAMP assays were validated with 25 serum samples from Cambodia

机译：背景技术通过考虑2056个全基因组DENV序列，开发了一种一步实时逆转录环介导的等温扩增（RT-LAMP）分析方法，用于检测登革热病毒（DENV）血清型。用来自坦桑尼亚，塞内加尔，苏丹和毛里塔尼亚的31份血液和11份血清样品验证了DENV1和DENV2 RT-LAMP分析的有效性。 DENV3和DENV4 RT-LAMP分析已用来自柬埔寨的25个血清样本进行了验证

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期刊名称 PLoS Neglected Tropical Diseases
作者
Benjamin Lopez-Jimena; Michaël Bekaert; Mohammed Bakheit; Sieghard Frischmann; Pranav Patel; Etienne Simon-Loriere; Louis Lambrechts; Veasna Duong; Philippe Dussart; Graham Harold; Cheikh Fall; Oumar Faye; Amadou Alpha Sall; Manfred Weidmann;
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年(卷),期 2018(12),5
年度 2018
页码 e0006381
总页数 22
原文格式 PDF
正文语种
中图分类寄生动物、寄生虫学;医学寄生虫学;
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1. Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype [J] . Benjamin Lopez-Jimena, Micha?l Bekaert, Mohammed Bakheit, PLOS Neglected Tropical Diseases . 2018,第5期

机译：研发和验证四种用于一步一步检测每种登革热病毒血清型的实时RT-LAMP检测方法
2. Development of Group- and Serotype-Specific One-Step SYBR Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus [J] . Pei-Yun Shu, Shu-Fen Chang, Yu-Chung Kuo, Journal of Clinical Microbiology . 2003,第6期

机译：基于组和血清型的一步法SYBR Green I实时登革热PCR方法的开发
3. Development and Validation of a Quantitative, One-Step, Multiplex, Real-Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses [J] . Monika Simmons, Todd Myers, Carolina Guevara, Journal of Clinical Microbiology . 2016,第7期

机译：登革热和基孔肯雅病毒检测的定量，一步，多重，实时逆转录酶PCR检测方法的开发和验证
4. Application of hydroxyapatite to detect specific antibodies -Anti-dengue virus IgM detection PA assay system approx [C] . Y. Kurosawa, A. Yamamoto, M. Nakayama Annual Meeting of The Ceramic Society of Japan . 2005

机译：羟基磷灰石检测特异性抗体的应用 - anti-dengue病毒IgM检测PA测定系统约
5. Development and use of a quantitative real-time PCR assay for rhinovirus serotype-16. [D] . Sachs, Lorne Allyn. 2010

机译：鼻病毒血清型16的实时定量PCR检测方法的开发和使用。
6. Development of Group- and Serotype-Specific One-Step SYBR Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus [O] . Pei-Yun Shu, Shu-Fen Chang, Yu-Chung Kuo, 2003

机译：基于组和血清型的一步法SYBR Green I登革热实时反转录PCR检测方法的开发
7. Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype [O] . Benjamin Lopez-Jimena, Michaël Bekaert, Mohammed Bakheit, 2018

机译：用于特异性检测每种登革病毒血清型的四步实时RT灯测定的开发和验证

Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype

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