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The International Society for Extracellular Vesicles launches the first massive open online course on extracellular vesicles

机译:国际细胞外囊泡学会启动了关于细胞外囊泡的首个大规模在线公开课程

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摘要

The International Society for Extracellular Vesicles (ISEV) has organised its first educational online course for students and beginners in the field of extracellular vesicles (EVs). This course, “Basics of Extracellular Vesicles,” uses recorded lectures from experts in the field and will be open for an unlimited number of participants. The course is divided into 5 modules and can be accessed at . The first module is an introduction to the field covering the nomenclature and history of EVs. Module 2 focuses on the biogenesis and uptake mechanisms of EVs, as well as their RNA, protein and lipid cargo. Module 3 covers the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Modules 4 and 5 present different isolation methods and characterisation techniques utilised in the EV field. Here, differential ultracentrifugation, size-exclusion chromatography, density gradient centrifugation, kit-based precipitation, electron microscopy, cryo-electron microscopy, flow cytometry, atomic-force microscopy and nanoparticle-tracking analysis are covered. This first massive open online course (MOOC) on EVs was launched on 15 August 2016 at the platform “Coursera” and is free of charge.
机译:国际细胞外囊泡学会(ISEV)为细胞外囊泡(EVs)领域的学生和初学者组织了首个在线教育课程。本课程“细胞外囊泡基础知识”使用了该领域专家录制的演讲,并且将对不限数量的参与者开放。该课程分为5个模块,可以在访问。第一个模块是介绍电动汽车的术语和历史的领域的简介。模块2重点介绍电动汽车的生物发生和摄取机制,以及它们的RNA,蛋白质和脂质运输。模块3涵盖了隔离电动汽车之前细胞培养基和体液(例如血液,母乳,脑脊髓液和尿液)的收集和处理。模块4和5展示了EV领域中使用的不同隔离方法和表征技术。在这里,涵盖了差速超速离心,尺寸排阻色谱,密度梯度离心,基于试剂盒的沉淀,电子显微镜,冷冻电子显微镜,流式细胞仪,原子力显微镜和纳米颗粒跟踪分析。该电动汽车首次大规模在线公开课程(MOOC)于2016年8月15日在“ Coursera”平台上免费开放。

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