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Universally applicable three-dimensional hydrodynamic microfluidic flow focusing

机译:普遍适用的三维流体动力学微流聚焦

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摘要

We have demonstrated a microfluidic device that can not only achieve three-dimensional flow focusing but also confine particles to the center stream along the channel. The device has a sample channel of smaller height and two sheath flow channels of greater height, merged into the downstream main channel where 3D focusing effects occur. We have demonstrated that both beads and cells in our device display significantly lower CVs in velocity and position distributions as well as reduced probability of coincidental events than they do in conventional 2D-confined microfluidic channels. The improved particle confinement in the microfluidic channel is highly desirable for microfluidic flow cytometers and in fluorescence-activated cell sorting (FACS). We have also reported a novel method to measure the velocity of each individual particle in the microfluidic channel. The method is compatible with the flow cytometer setup and requires no sophisticated visualization equipment. The principles and methods of device design and characterization can be applicable to many types of microfluidic systems.
机译:我们已经证明了一种微流体装置,该装置不仅可以实现三维流聚焦,而且还可以将颗粒沿着通道限制在中心流中。该设备具有一个较小高度的样品通道和两个较大高度的鞘流通道,并合并到下游主通道中,在此发生3D聚焦效果。我们已经证明,与传统的二维受限微流控通道相比,设备中的磁珠和细胞在速度和位置分布上均显示出更低的CV,同时发生事件的可能性也大大降低。对于微流体流式细胞仪和荧光激活细胞分选术(FACS),非常需要在微流体通道中改善颗粒限制。我们还报告了一种新颖的方法来测量微流体通道中每个单独的粒子的速度。该方法与流式细胞仪的设置兼容,不需要复杂的可视化设备。装置设计和表征的原理和方法可以适用于许多类型的微流体系统。

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