Adipocyte reporter assays: Application for identification of anti-inflammatory and antioxidant properties of mangostin xanthones

摘要

ScopeThree fluorescence biosensors were developed based on a 3T3-L1 preadipocyte line that stably expressed Nfkb-RE/GFP, Fabp4-P/CFP, and Nrf2-P/YFP fluorescent reporters. We hypothesized that nutraceuticals’ inflammatory, adipogenic, and antioxidant status will be identified based on the change in fluorescence in reporter adipocytes. We validated these assays with activators of NFκB, FABP4-regulating PPARγ, NFR2 and, thereafter, tested known and unknown properties of mangosteens (MG), the xanthone metabolites in mangosteen fruit.