Predicting storage-dependent damage to Red Blood Cells using nitrite oxidation kinetics Peroxiredoxin-2 oxidation and hemoglobin / free heme measurements

摘要

BackgroundStorage-dependent damage to RBCs varies significantly. Identifying RBC units that will undergo higher levels of hemolysis during storage may allow for more efficient inventory management decision-making. Oxidative-stress mediates storage-dependent damage to RBCs and will depend on the oxidant : antioxidant balance. We reasoned that this balance or redox tone will serve as a determinant of how a given RBC unit stores and that its assessment in “young” RBC will predict storage-dependent hemolysis.