Single-Reaction Multiplex Real-Time RT-PCR for the Detection Quantitation and Serotyping of Dengue Viruses

摘要

BackgroundDengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for the detection, quantitation, and serotyping of dengue viruses in a single reaction.