DNA-encoded chemical libraries (DECLs) are collections of organic compounds, which are individually linked to different oligonucleotides, serving as amplifiable identification barcodes. Since all compounds in the library can be identified by their DNA tag, they can be mixed and used in affinity capture experiments on target proteins of interest. In this protocol, we describe the screening process that allows the identification of the few binding molecules within the multiplicity of library members. First, the automated affinity selection process physically isolates binding library members. Second, the DNA codes of the isolated binders are PCR-amplified and subjected to high-throughput DNA sequencing. Third, the obtained sequencing data are evaluated using a C++ program and the results displayed using MATLAB software. The resulting selection fingerprints facilitate the discrimination of binding from non-binding library members. The described procedures allow the identification of small organic ligands to biological targets from a DECL within 10 days.
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