Ion Mobility-Mass Spectrometry Analysis of Crosslinked Intact Multiprotein Complexes: Enhanced Gas-phase Stabilities and Altered Dissociation Pathways

摘要

Analysis of protein complexes by ion mobility-mass spectrometry is a valuable method for the rapid assessment of complex composition, binding stoichiometries, and structures. However, capturing labile, unknown protein assemblies directly from cells remains a challenge for the technology. Furthermore, ion mobility-mass spectrometry measurements of complexes, subcomplexes, and subunits are necessary to build complete models of intact assemblies, and such data can be difficult to acquire in a comprehensive fashion. Here, we present the use of novel mass spectrometry cleavable crosslinkers and tags to stabilize intact protein complexes for ion mobility-mass spectrometry. Our data reveal that tags and linkers bearing permanent charges are superior stabilizers relative to neutral crosslinkers, especially in the context of retaining compact forms of the assembly under a wide array of activating conditions. In addition, when cross-linked protein complexes are collisionally activated in the gas-phase, a larger proportion of the product ions produced are often more compact and reflect native protein sub-complexes when compared with unmodified complexes activated in the same fashion, greatly enabling applications in structural biology.