Competitive Adsorption of Plasma Proteins using Quartz Crystal Microbalance

摘要

Proteins that get adsorbed onto the surfaces of biomaterials immediately upon their implantation mediate the interactions between the material and the environment. This process, in which proteins in a complex mixture compete for adsorption sites on the surface, is determined by the physicochemical interactions at the interface. Competitive adsorption of bovine serum albumin (BSA), fibronectin (Fn), and collagen type I (Col I), sequentially and from mixtures, was investigated so as to understand the performances of different surfaces used in biomedical applications. Quartz crystal microbalance with dissipation was used to monitor the adsorption of these proteins onto two materials used in functional bone replacement, a titanium alloy (Ti6Al4V) and Ti6Al4V physisorbed with poly(sodium styrene sulfonate) (poly(NaSS)), and three controls, gold, poly(desaminotyrosyl-tyrosine ethyl ester carbonate) (poly(DTEc)) and polystyrene (PS). In experiments with individual proteins, the adsorption was the highest with Fn and Col I and the least with BSA. And, protein adsorption was the highest on poly(NaSS) and Ti6Al4V, and the least on poly(DTEc). In sequential adsorption experiments, protein exchange was observed in BSA + Fn, Fn + Col I and BSA + Col I sequences, but not in Fn + BSA and Col I + BSA due to the lower affinity of BSA to surfaces relative to Fn and Col I. Protein adsorption was the highest with Col I + Fn on hydrophobic surfaces. In experiments with protein mixtures, with BSA & Fn, Fn appears to be preferentially adsorbed; with Fn & Col I, both proteins were adsorbed, probably as multilayers; and with Col I & BSA, the total amount of protein was the highest, greater than in sequential and individual adsorption of the two proteins, probably due to the formation of BSA and Col I complexes. Protein conformational changes induced by the adsorbing surfaces, protein-protein interactions, affinities of proteins appear to be the important factors that govern competitive adsorption. The findings reported here will be useful in understanding host response to surfaces used for implants.