PR1-specific cytotoxic T lymphocytes are relatively frequent in umbilical cord blood and can be effectively expanded to target myeloid leukemia

摘要

Background aimsPR1 is an HLA-A2 restricted leukemia-associated antigen derived from neutrophil elastase and proteinase 3, both of which are normally stored in the azurophil granules of myeloid cells but overexpressed in myeloid leukemic cells. PR1-specific cytotoxic lymphocytes (PR1-CTL) have activity against primary myeloid leukemia in vitro and in vivo and thus could have great potential in the setting of adoptive cellular therapy (ACT). Adult peripheral blood derived PR1-CTL are infrequent but preferentially lyse myeloid leukemia cells. We sought to examine PR1-CTL in umbilical cord blood (UCB) since UCB units provide a rapidly available cell source and a lower risk of graft-versus-host disease (GVHD), even in the setting of mismatched human leukocyte antigen (HLA) loci.