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Isolation and genomic characterization of six endophytic bacteria isolated from Saccharum sp (sugarcane): Insights into antibiotic secondary metabolite and quorum sensing metabolism

机译:分离自蔗糖属(蔗糖)的六种内生细菌的分离和基因组学表征:深入了解抗生素次生代谢产物和群体感应代谢

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摘要

Six endophytic bacteria were isolated from Saccharum sp (sugarcane) grown in the parish of Westmoreland on the island of Jamaica located in the West Indies. Whole genome sequence and annotation of the six bacteria show that three were from the genus Pseudomonas and the other three were from the genera Pantoea, Pseudocitrobacter, and Enterobacter. A scan of each genome using the antibiotics and secondary metabolite analysis shell (antiSMASH4.0) webserver showed evidence that the bacteria were able to produce a variety of secondary metabolites. In addition, we were able to show that one of the organisms, Enterobacter sp RIT418 produces N-acyl-homoserine lactones (AHLs), which is indicative of cell-cell communication via quorum sensing (QS).
机译:从生长在西印度群岛牙买加岛威斯特摩兰教区的蔗糖(蔗糖)中分离出六种内生细菌。六种细菌的全基因组序列和注释显示,其中三种来自假单胞菌属,另外三种来自泛菌,假单胞菌属和肠杆菌属。使用抗生素和次级代谢产物分析外壳(antiSMASH4.0)网络服务器对每个基因组进行扫描,显示出该细菌能够产生多种次级代谢产物的证据。此外,我们能够证明一种细菌,即Enterobacter sp RIT418会产生N-酰基-高丝氨酸内酯(AHL),这表明通过群体感应(QS)进行细胞间通讯。

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