High Concordance of a Closed-System RT-qPCR Breast Cancer Assay for HER2 mRNA Compared to Clinically Determined Immunohistochemistry Fluorescence in situ Hybridization and Quantitative Immunofluorescence

摘要

BackgroundHistorically, mRNA measurements have been tested on several commercially available platforms, but none have gained broad acceptance for assessment of HER2. An mRNA measurement, as a continuous value, has the potential for use in adjudication of the equivocal category. Here we use a real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay in a closed, single-use cartridge, automated system.