Real-Time Monitoring of the Aggregation of Alzheimer’s Amyloid-β by 1H Magic Angle Spinning NMR Spectroscopy

摘要

Proton magic-angle-spinning NMR used for real-time analysis of amyloid aggregation reveals that the mechanical rotation of Aβ1–40 monomers increases the rate of formation of aggregates, and that the increasing lag-time with peptide concentration suggests the formation of growth-incompetent species. EGCG’s ability to shift off-pathway aggregation is also demonstrated.