In-Capillary Microextraction for Direct Mass Spectrometry Analysis of Biological Samples

摘要

Slug flow microextraction-nanoESI (SFME-nanoESI) was originally explored as a single-step sampling ionization method for MS analysis of biofluids. In this work, a comprehensive study and development of the SFME has been carried out. Revers-phase SFME was developed to analyze chemical compounds in oil samples. A three-phase SFME system was introduced as a suitable approach for analyzing polar compounds in biofluids. The impacts by the capillary inner diameter, solvent and sample properties were also investigated, leading the use of fused silica capillaries for performing SFME.