Fluorescence Sensing Approach for High Specific Detectionof 246-Trinitrophenol Using Bright Cyan Blue Color-Emittive Poly(vinylpyrrolidone)-SupportedCopper Nanoclusters as a Fluorophore

摘要

In this paper, we illustrate an efficient, convenient, and simplistic fluorescence technique for the specific identification for nitro explosive 2,4,6-trinitrophenol (TNP) in 100% water medium by bright cyan blue color-emitting poly(vinylpyrrolidone)-supported copper nanoclusters (PVP-CuNCs) as a fluorescence probe. PVP-CuNCs exhibited linear fluorescence quenching response toward the increasing concentration of TNP analyte. Surprisingly, TNP only reduces the emission signal of PVP-CuNCs, whereas various nitro explosives cause very slight reducing emission intensity, validating the good specificity of the PVP-CuNC probe toward TNP. The highest Stern–Volmer quenching constant (Ksv) value of 1.03 × 10⁷ dm³ mol^–1 and the extremely lowest limit of detection of 81.44 × 10^–12 mol dm^–3 were achieved solely for TNP in 100% water medium which is astonishing and exclusive for this nanoprobe. The sensing pathway for the high sensitivity of PVP-CuNCs assay to quantify the TNP is expected to combine with the inner filter effect process and static quenching. The static quenching mechanism between TNP and PVP-CuNCs is further verified by fluorescence decay measurements. Furthermore, the developedfluorescence sensing platform is applied for the quantification ofa trace amount of TNP in real samples named dam water, sea water,and match stick.