Are microsatellite fragment lengths useful for population-level studies? The case of Polygala lewtonii (Polygalaceae)

摘要

Premise of the study:Microsatellites, despite being commonly used population-level markers, contain biases because scoring relies solely on fragment length. Their complexity can lead to homoplasy, the effects of which are poorly understood. We investigated the impact of using fragment lengths, repeats, or flanking region sequences on common population-level analyses.