Muscle ion transporters and antioxidative proteins have different adaptive potential in arm than in leg skeletal muscle with exercise training

摘要

It was evaluated whether upper‐body compared to lower‐body musculature exhibits a different phenotype in relation to capacity for handling reactive oxygen species (ROS), H⁺, La⁻, Na⁺, K⁺ and also whether it differs in adaptive potential to exercise training. Eighty‐three sedentary premenopausal women aged 45 ± 6 years (mean ± SD) were randomized into a high‐intensity intermittent swimming group (HIS, n = 21), a moderate‐intensity swimming group (MOS, n = 21), a soccer group (SOC, n = 21), or a control group (CON, n = 20). Intervention groups completed three weekly training sessions for 15 weeks, and pre‐ and postintervention biopsies were obtained from deltoideus and vastus lateralis muscle. Before training, monocarboxylate transporter 4 (MCT4), Na⁺/K⁺ pump α 2, and superoxide dismutase 2 (SOD2) expressions were lower (P < 0.05) in m. deltoideus than in m. vastus lateralis, whereas deltoid had higher (P < 0.05) Na⁺/H⁺ exchanger 1 (NHE1) expression. As a result of training, Na⁺/K⁺ pump α 2 isoform expression was elevated only in deltoideus muscle, while upregulation (P < 0.05) of the α 1 and β 1 subunits, phospholemman (FXYD1), NHE1, and superoxide dismutase 1 expression occurred exclusively in vastus lateralis muscle. The increased (P < 0.05) expression of MCT4 and SOD2 in deltoid muscle after HIS and vastus lateralis muscle after SOC were similar. In conclusion, arm musculature displays lower basal ROS, La⁻, K⁺ handling capability but higher Na⁺‐dependent H⁺ extrusion capacity than leg musculature. Training‐induced changes in the ion‐transporting and antioxidant proteins clearly differed between muscle groups.