LncRNA HOTAIR influences cell growth migration invasion and apoptosis via the miR‐20a‐5p/HMGA2 axis in breast cancer

摘要

To study the regulatory effect of lncRNA HOTAIR/miR‐20a‐5p/HMGA2 axis on breast cancer (BC) cell growth, cell mobility, invasiveness, and apoptosis. The microarray data of lncRNAs and mRNAs with differential expression in BC tissues were analyzed in the Cancer Genome Atlas (TCGA) database. LncRNA HOX transcript antisense RNA (lncRNA HOTAIR) expression in BC was assessed by qRT‐PCR. Cell viability was confirmed using MTT and colony formation assay. Cell apoptosis was analyzed by TdT‐mediated dUTP nick‐end labeling (TUNEL) assay. Cell mobility and invasiveness were testified by transwell assay. style="fixed-case">RNA pull‐down and dual luciferase assay were used for analysis of the correlation between lnc style="fixed-case">RNA HOTAIR and miR‐20a‐5p, as well as relationship of miR‐20a‐5p with high mobility group style="fixed-case">AT‐hook 2 ( style="fixed-case">HMGA2). Tumor xenograft study was applied to confirm the correlation of lnc style="fixed-case">RNA HOTAIR/miR‐20a‐5p/ style="fixed-case">HMGA2 axis on style="fixed-case">BC development in vivo. The expression levels of the lnc style="fixed-case">RNA HOTAIR were upregulated in style="fixed-case">BC tissues and cells. Knockdown lnc style="fixed-case">RNA HOTAIR inhibited cell propagation and metastasis and facilitated cell apoptosis. MiR‐20a‐5p was a target of lnc style="fixed-case">RNA HOTAIR and had a negative correlation with lnc style="fixed-case">RNA HOTAIR. MiR‐20a‐5p overexpression in style="fixed-case">BC suppressed cell growth, mobility, and invasiveness and facilitated apoptosis. style="fixed-case">HMGA2 was a target of miR‐20a‐5p, which significantly induced carcinogenesis of style="fixed-case">BC. style="fixed-case">BC cells progression was mediated by lnc style="fixed-case">RNA HOTAIR via affecting miR‐20a‐5p/ style="fixed-case">HMGA2 in vivo. Lnc style="fixed-case">RNA HOTAIR affected cell growth, metastasis, and apoptosis via the miR‐20a‐5p/ style="fixed-case">HMGA2 axis in breast cancer.