A metabolomic study of the PPARδ agonist GW501516 for enhancing running endurance in Kunming mice

摘要

Exercise can increase peroxisome proliferator-activated receptor-δ (PPARδ) expression in skeletal muscle. PPARδ regulates muscle metabolism and reprograms muscle fibre types to enhance running endurance. This study utilized metabolomic profiling to examine the effects of , a PPARδ agonist, on running endurance in mice. While training alone increased the exhaustive running performance, treatment enhanced running endurance and the proportion of succinate dehydrogenase (SDH)-positive muscle fibres in both trained and untrained mice. Furthermore, increased levels of intermediate metabolites and key enzymes in fatty acid oxidation pathways were observed following training and/or treatment. Training alone increased serum inositol, glucogenic amino acids, and branch chain amino acids. However, increased serum galactose and β-hydroxybutyrate, independent of training. Additionally, alone raised serum unsaturated fatty acid levels, especially polyunsaturated fatty acids, but levels increased even more when combined with training. These findings suggest that mechanisms behind enhanced running capacity are not identical for and training. Training increases energy availability by promoting catabolism of proteins, and gluconeogenesis, whereas enhances specific consumption of fatty acids and reducing glucose utilization.