A millisecond micro-RNA separation of a mixture of total RNA and genomic DNA, extracted from cultured HeLa cells, was successfully achieved using a hybrid structure of nanopillars and nanoslits contained inside a microchannel. The nanopillars, 250-nm in diameter and 100-nm in height, were fabricated with a 750-nm space inside the nanoslits, which were 100-nm in height and 25-μm in width; the nanopillars were then applied as a new sieve matrix. This ultra-fast technique for the separation of miRNA can be an effective pretreatment for semiconductor nanopore DNA sequencing, which has an optimum reading speed of 1 base/ms to obtain effective signal-to-noise ratio and discriminate each base by ion or tunneling current during the passage of nucleic acids.
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机译:使用微通道内包含的纳米柱和纳米缝的混合结构,成功地实现了从培养的HeLa细胞提取的总RNA和基因组DNA混合物的毫秒级微RNA分离。纳米柱的直径为250nm,高度为100nm,在纳米缝内部留有750nm的空间,纳米柱的高度为100nm,宽度为25μm。然后将纳米柱用作新的筛基。这种用于miRNA分离的超快速技术可以作为半导体纳米孔DNA测序的有效预处理,其最佳读取速度为1 base / ms,以获得有效的信噪比并通过离子或隧道电流区分每个碱基在核酸通过过程中。
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