Phragmites australis (an invasive plant in North America) was used as feedstock for ABE (acetone-butanol-ethanol) fermentation by Clostridium saccharobutylicum. Sulphuric acid pretreated phragmites hydrolysate (SAEH) without detoxification inhibited butanol production (0.73 g/L butanol from 30 g/L sugars). The treatment of SAEH with resin L-493 prior the fermentation resulted in no inhibitory effects and an ABE titer of 14.44 g/L, including 5.49 g/L butanol was obtained, corresponding to an ABE yield and productivity of 0.49 g/g and 0.60 g/L/h, respectively. Dual functionality of the resin was realized by also using it as an in-situ product removal agent. Integrating in-situ product removal allowed for the use of high substrate concentrations without the typical product inhibition. Resin-detoxified SAEH was supplemented with neat glucose and an effective ABE titer of 33 g/L (including 13.7 g/L acetone, 16.4 g/L butanol and 1.9 g/L ethanol) was achieved with resin-based in-situ product removal, corresponding to an ABE yield and productivity of 0.41 g/g and 0.69 g/L/h, respectively. Both detoxification of the substrate and the products was achieved by the same resin, which was added prior the fermentation. Integrating hydrolysate detoxification and in-situ butanol removal in a batch process through single resin can potentially simplify cellulosic butanol production.
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机译:芦苇(北美的一种入侵植物)被用作糖丁酸梭菌发酵的ABE(丙酮-丁醇-乙醇)发酵的原料。硫酸预处理的芦苇水解产物(SAEH)无需解毒,可抑制丁醇的产生(从30μg/ L的糖中生成0.73μg/ L的丁醇)。发酵前用树脂L-493对SAEH的处理没有抑制作用,得到的ABE滴度为14.44 / g / L,其中丁醇的含量为5.49 g / L,对应的ABE收率和产率分别为0.49 g / g和0.60 g / L / h。树脂的双重功能也可以通过将其用作原位产品去除剂来实现。集成的原位产物去除技术允许使用高浓度的底物,而不会产生典型的产物抑制作用。树脂解毒的SAEH补充了纯净的葡萄糖,去除了基于树脂的原位产物后,有效的ABE滴度达到33 g / L(包括13.7μg/ L丙酮,16.4μg/ L丁醇和1.9μg/ L乙醇)。 ,对应的ABE产量和生产率分别为0.41μg/ g和0.69μg/ L / h。底物和产物的解毒均通过相同的树脂实现,该树脂在发酵之前添加。通过单一树脂将水解产物的解毒和原位丁醇去除合并在一起可以潜在地简化纤维素丁醇的生产。
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