MicroRNA-218 inhibits the migration epithelial-mesenchymal transition and cancer stem cell properties of prostate cancer cells

摘要

MicroRNA (miRNA) is a class of non-coding single-stranded RNA, able to regulate tumor-associated genes via binding the 3′-UTR of the target gene mRNA. Previous publications have demonstrated that miRNA-218 (miR-218) acts as a tumor-suppressive miRNA in various types of human cancer, including prostate cancer (PCa). However, the role of miR-218 in regulating PCa cell stemness and epithelial-mesenchymal transition remains unknown and requires further research. In the present study, it is demonstrated that miR-218 was downregulated in 2 PCa cell lines and could suppress cell migration, EMT and the exhibition of cancer stem cell-like properties. The expression of GLI family zinc finger 1 (Gli1) was inhibited by miR-218 overexpression, suggesting miR-218-suppression of Gli1 as a potential mechanism for the tumor-suppressive effect of miR-218. Overall, the results indicate that miR-218 served a critical role in the inhibition of PCa development. This may provide new insight for elucidating the mechanisms of PCa oncogenesis and suggests that miR-218 may be a novel therapeutic target for PCa.