Intravenous injection of post-hemorrhagic shock mesenteric lymph induces multiple organ injury in rats

摘要

Post-hemorrhagic shock mesenteric lymph (PHSML) has an important role in the multiple organ injuries caused by severe shock. The current study investigated whether intravenous injection of PHSML induces organ injury in normal rats. Following the establishment of hemorrhagic shock in donor rats (40±2 mmHg, 3 h), PHSML was drained during hypotension at 1–3 h and then injected to normal rats through the femoral vein within 30 min. The mean arterial pressure (MAP) was measured, and samples were obtained for analysis of histology and biochemical indices at 2.5 h post-PHSML administration. PHSML administration resulted in a significant decrease in MAP at the early and late stage of the experiment. Structural damage of the lung, kidney, heart and liver was also observed, and the levels of urea, creatinine, aspartate aminotransferase, total bile acid and creatine kinase MB isoenzyme were increased in the plasma. Additionally, PHSML injection significantly increased the levels of trypsin, tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 and receptor of advanced glycation end-products in the plasma, malondialdehyde in the lung and myocardium, and TNF-α in the lung, kidney, myocardium and liver. Intravenous injection of PHSML induced multiple organ injury in normal rats via increases in trypsin activity, inflammatory factors and free radical production. The findings indicate that PHSML return is an important contributor to organ damage following hemorrhagic shock.