The Role of Dynamin in HIV Type 1 Env-Mediated Cell–Cell Fusion

摘要

HIV-1 envelope glycoproteins are the key viral proteins that mediate HIV-1 entry and cell–cell fusion. In contrast to HIV-1 entry, the mechanism of HIV-1 Env-mediated cell–cell fusion is relatively unclear. This study demonstrated that dynasore, a dynamin inhibitor, suppressed HIV-1 Env-mediated cell–cell fusion. Dynasore sensitivity of HIV-1 Env-mediated cell–cell fusion varied depending on the viral strains. Results from testing a panel of gp41 cytoplasmic tail truncation mutants suggested that the gp41 cytoplasmic tail might play a role in dynasore sensitivity. HIV-1 Env-mediated cell–cell fusion could also be suppressed by a dynamin dominant-negative mutant DNM2(K44A). In summary, these results suggested that dynamin 2 might play a role in HIV-1 Env-mediated cell–cell fusion.