Substitution of Malachite Green with Nigrosin - Eosin Yellow Stain in the Kato-Katz method: microscopical appearance of the helminth eggs

摘要

BackgroundThe Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections. The major problem of the technique is that a few hours after the preparation of slides hookworm eggs over clear and disappear due glycerin.