Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis for Rapid Confirmation of Burkholderia pseudomallei in Septicemic Melioidosis

摘要

Burkholderia pseudomallei was quickly identified from blood cultures collected from septicemic patients by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis using an in-house reference library. This procedure reduced the time to definitive identification by more than 24 hours. This analysis is a useful addition to laboratory methods for early recognition of septicemic melioidosis in non-endemic settings.