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Discovery of Plant Phenolic Compounds That Act as Type III Secretion System Inhibitors or Inducers of the Fire Blight Pathogen Erwinia amylovora

机译:发现作为火疫病病原体欧文氏小球藻的III型分泌系统抑制剂或诱导物的植物酚类化合物

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摘要

Erwinia amylovora causes a devastating disease called fire blight in rosaceous plants. The type III secretion system (T3SS) is one of the important virulence factors utilized by E. amylovora in order to successfully infect its hosts. By using a green fluorescent protein (GFP) reporter construct combined with a high-throughput flow cytometry assay, a library of phenolic compounds and their derivatives was studied for their ability to alter the expression of the T3SS. Based on the effectiveness of the compounds on the expression of the T3SS pilus, the T3SS inhibitors 4-methoxy-cinnamic acid (TMCA) and benzoic acid (BA) and one T3SS inducer, trans-2-(4-hydroxyphenyl)-ethenylsulfonate (EHPES), were chosen for further study. Both the T3SS inhibitors (TMCA and BA) and the T3SS inducer (EHPES) were found to alter the expression of T3SS through the HrpS-HrpL pathway. Additionally, TMCA altered T3SS expression through the rsmBEa-RsmAEa system. Finally, we found that TMCA and BA weakened the hypersensitive response (HR) in tobacco by suppressing the T3SS of E. amylovora. In our study, we identified phenolic compounds that specifically targeted the T3SS. The T3SS inhibitor may offer an alternative approach to antimicrobial therapy by targeting virulence factors of bacterial pathogens.
机译:淀粉欧文氏杆菌在玫瑰科植物中引起毁灭性疾病,称为火疫病。 III型分泌系统(T3SS)是淀粉双歧杆菌用于成功感染其宿主的重要毒力因子之一。通过使用绿色荧光蛋白(GFP)报告基因构建体与高通量流式细胞术分析相结合,研究了酚类化合物及其衍生物的文库改变T3SS表达的能力。根据化合物对T3SS菌毛表达的有效性,T3SS抑制剂4-甲氧基肉桂酸(TMCA)和苯甲酸(BA)和一种T3SS诱导剂反式2-(4-羟苯基)-乙烯基磺酸盐( EHPES),被选作进一步研究。发现T3SS抑制剂(TMCA和BA)和T3SS诱导剂(EHPES)都通过HrpS-HrpL途径改变T3SS的表达。此外,TMCA通过rsmBEa-RsmAEa系统改变了T3SS表达。最后,我们发现TMCA和BA通过抑制淀粉样芽胞杆菌的T3SS减弱了烟草中的超敏反应(HR)。在我们的研究中,我们确定了专​​门针对T3SS的酚类化合物。通过靶向细菌病原体的毒力因子,T3SS抑制剂可为抗菌治疗提供替代方法。

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