Estimation of Campylobacter spp. in broth culture by bioluminescence assay of ATP.

摘要

The luciferin-luciferase bioluminescence reaction was used to estimate cell numbers of Campylobacter jejuni and Campylobacter coli in broth cultures based on a linear relationship between cell numbers (in excess of 10(4) to 10(5] and ATP levels. The sensitivity was lower than that obtained with Escherichia coli. The calculated amount of intracellular ATP per cell of C. jejuni and C. coli ranged from 1.7 to 2.1 fg.