A chemically defined medium, LD82, was formulated for in vitro cultivation of spiroplasmas. Medium LD82 supported good growth for four epiphytic and insect-pathogenic spiroplasmas, Spiroplasma floricola 23-6T, Spiroplasma sp. strain SR3, Spiroplasma sp. strain brevi, and Spiroplasma sp. strain AS576, and of the phytopathogenic spiroplasmas Spiroplasma citri Maroc R8A2T and PC1. Titers of all six strains grown in defined medium LD82 reached 2.0 × 109 to 6.0 × 109 CFU/ml of culture. All spiroplasma strains tested formed colonies readily on agar medium LD82. None of the spiroplasmas formed typical fried-egg colonies. All formed diffuse colonies, but the forms of colonies differed somewhat among the spiroplasma strains. In preliminary studies of nutritional requirements, phospholipids slightly enhanced the growth of the epiphytic and insect-pathogenic strains in medium LD82 and were found essential for good growth of S. citri.
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机译：配制了化学定义的培养基LD82，用于螺旋体的体外培养。 LD82中等培养基对四个附生和昆虫致病性螺旋体，小螺旋体23-6 T 和螺旋体sp。都有良好的生长。菌株SR3，Spiroplasma sp。菌株brevi和Spiroplasma sp。株AS576，以及植物致病性螺旋体，柠檬螺旋体Maroc R8A2 T 和PC1。在限定培养基LD82中生长的所有六种菌株的滴度达到2.0×10 9 至6.0×10 9 CFU / ml培养物。所有测试的螺旋体菌株均在琼脂培养基LD82上容易地形成菌落。没有一个螺旋体形成典型的煎蛋菌落。所有形成的散布菌落，但在螺旋体菌株之间菌落的形式有所不同。在营养需求的初步研究中，磷脂稍微促进了培养基LD82中附生和昆虫致病菌株的生长，并发现其对于柠檬葡萄球菌的良好生长必不可少。
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