A procedure is described for the purification of hemagglutinin-free Clostridium botulinum type C toxin. The toxin was purified approximately 1,000-fold from the original culture supernatant in an overall yield of 60% to a final specific toxicity of 4.4 x 10(7) minimal lethal doses/mg of protein. The toxin had a molecular weight of 141,000 and consisted of a heavy and a light chain. The molecular weights of the subunits were approximately 98,000 and 53,000. When comparing the molecular size and composition of type C toxin to that of botulinum toxins of different types, some common features may be suggested; i.e., the toxin has a molecular weight between 141,000 to 160,000 and is comprised of a heavy and a light chain linked by disulfide bonds (or bond).
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机译：描述了纯化无血凝素的C型肉毒梭状芽孢杆菌毒素的程序。从原始培养物上清液中纯化毒素约1000倍，总产率为60％，最终毒性为4.4 x 10（7）最小致死剂量/ mg蛋白。该毒素的分子量为141,000，由重链和轻链组成。亚基的分子量约为98,000和53,000。当将C型毒素与不同类型的肉毒杆菌毒素的分子大小和组成进行比较时，可能会提出一些共同的特征。即，该毒素的分子量在141,000至160,000之间，并且由通过二硫键（或键）连接的重链和轻链组成。
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