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Effects of Time and Growth Media on Short-Chain Fatty Acid Production by Bacteroides fragilis

机译:时间和生长培养基对脆弱拟杆菌的短链脂肪酸生产的影响

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摘要

Gas-liquid chromatography was used to monitor the evolution of short chain fatty acids by Bacteroides fragilis in five media. Acetic and succinic acids, the prominent end products encountered, were readily detected within 24 h. Propionic, isobutyric, butyric, isovaleric, and lactic acids were usually recorded in more limited quantities. Maximum rates of bacterial multiplication, glucose catabolism, and end-product production coincided with the first 24 h in carbohydrate-supplemented media. Extended incubation (672 h) favored substantial succinate increases in three of five media. These observations suggest that incubation time and composition of the medium are important determinants in short chain fatty acid production by B. fragilis.
机译:气液色谱法用于监测脆弱细菌在5种培养基中短链脂肪酸的演变。乙酸和琥珀酸是遇到的主要终产物,很容易在24小时内检测到。通常记录的丙酸,异丁酸,丁酸,异戊酸和乳酸的含量更为有限。在补充碳水化合物的培养基中,最初的24小时细菌繁殖,葡萄糖分解代谢和终产物产生的最大速率相吻合。延长孵育时间(672小时)有利于琥珀酸在5种培养基中的3种中大量增加。这些观察结果表明,温育时间和培养基组成是脆弱芽孢杆菌产生短链脂肪酸的重要决定因素。

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