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Evaluation of Miniature Test for Bacteriuria Using Dehydrated Media and Nitrite Pads

机译:使用脱水培养基和亚硝酸盐垫对细菌性尿症进行微型试验的评估

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摘要

A new dip-inoculum method for detecting bacteriuria which utilizes dehydrated media pads and a nitrite pad attached to a small plastic strip was evaluated in hospitalized patients. Discrepant interpretations were made by independent observers in 9.3% of the specimens with > 105 colonies per ml. The media pads failed to support growth of yeast and gave variable results with Staphylococcus epidermidis and non-group D streptococci. False-negative culture results commonly occurred if the patients were receiving antibiotics. The nitrite test occasionally remained positive for brief periods after the elimination of bacteriuria by antibiotics. Conditions and drugs (especially phenazopyridine) which discolor urine interfered with reading both the culture and nitrite tests. Although not suitable for hospital use, or for monitoring therapy, the test strip is probably as reliable as the calibrated loop-streak plate culture for office screening.
机译:在住院患者中,评估了一种新的浸没菌检测细菌尿的方法,该方法利用了脱水培养基垫和附着在小塑料条上的亚硝酸盐垫。独立观察者对9.3%的标本(每毫升> 10 5 )的菌落做出了不同的解释。培养基垫不能支持酵母的生长,并且对表皮葡萄球菌和非D组链球菌产生可变的结果。如果患者正在使用抗生素,通常会出现假阴性培养结果。在通过抗生素消除尿尿后,亚硝酸盐测试偶尔会在短时间内保持阳性。使尿液变色的条件和药物(尤其是苯并吡啶)会干扰培养和亚硝酸盐检测的读数。尽管不适合用于医院或监测治疗,但该试纸条的可靠性可能与用于办公室筛查的校准环纹板培养物一样可靠。

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