In Kenya, leaves and roots from Croton macrostachyus are used as a traditional medicine for infectious diseases such as typhoid and measles, but reports on possible antimicrobial activity of stem bark do not exist. In this study, the antibacterial and antifungal effects of methanol, ethyl acetate and butanol extracts, and purified lupeol of C. macrostachyus stem bark were determined against important human gram-negative pathogens Escherichia coli, Salmonella typhi, Klebsiella pneumoniae, and Enterobacter aerogenes, gram-positive Listeria monocytogenes, and a fungus Candida albicans. The most promising broad scale antimicrobial activity against all the studied pathogens was shown by the ethyl acetate extract. The ethyl acetate extract induced the zone of inhibition between 10.1 ± 0.6 mm and 16.0 ± 1.2 mm against S. typhi, E. coli, K. pneumoniae, E. aerogenes, and L. monocytogenes with weaker antimicrobial activity against C. albicans (zone of inhibition: 5.6 ± 1.0 mm). The antibiotic controls (amoxicillin, ciprofloxacin, ampicillin, benzylpenicillin, clotrimazole, and cefotaxime) showed antimicrobial activity with zones of inhibition within 13.4 ± 0.7–22.1 ± 0.9 mm. The ethyl acetate extract had MIC in the range of 125–250 mg/mL against all the studied bacteria and against C. albicans MIC was 500 mg/mL. The present results give scientific evidence and support the traditional use of C. macrostachyus stem bark as a source for antimicrobials. We show that C. macrostachyus stem bark lupeol is a promising antimicrobial agent against several important human pathogens.
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机译:在肯尼亚,巴豆大叶的叶和根被用作伤寒和麻疹等传染病的传统药物,但没有关于茎皮可能具有抗菌活性的报道。在这项研究中,测定了甲醇,乙酸乙酯和丁醇提取物以及纯化的大水梭菌茎皮的卢比欧尔对重要的人类革兰氏阴性病原体大肠杆菌,鼠伤寒沙门氏菌,肺炎克雷伯菌和产气肠杆菌的抗菌和抗真菌作用。阳性单核细胞增生性李斯特菌和真菌白色念珠菌。乙酸乙酯提取物显示出对所有已研究病原体最有前途的大规模抗菌活性。乙酸乙酯提取物诱导对伤寒链球菌,大肠杆菌,肺炎克雷伯菌,产气链球菌和单核细胞增生李斯特菌的抑制作用范围在10.1±0.6?mm和16.0±1.2?mm之间(对白色念珠菌的抗菌作用较弱)抑制系数:5.6±1.0 mm)。抗生素对照(阿莫西林,环丙沙星,氨苄青霉素,苄青霉素,克霉唑和头孢噻肟)显示抗菌活性,其抑制范围在13.4±0.7–22.1±0.9 mm之内。乙酸乙酯提取物对所有研究细菌和白色念珠菌的MIC在125-250μmg/ mL范围内,MIC为500μmg/ mL。目前的结果提供了科学依据,并支持传统的大花梭菌茎皮作为抗菌素的来源。我们表明, C. macrostachyus em>茎皮鲁皮醇是针对几种重要的人类病原体的有前途的抗菌剂。
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