1. A sequence of 73 nucleotides of the RNA genome from coliphage R17 was determined. It can be read through in only one translational frame. The fragment is not part of the coatprotein cistron (Min Jou et al., 1972), nor does it come from the untranslated sequences described previously (Steitz, 1969; Nichols, 1970; Cory et al., 1970; de Wachter et al., 1971; Contreras et al., 1971; Cory et al., 1972). It contains two sequences of 23 and 24 nucleotides, 22 of which are identical. This kind of reiteration is the first one found in bacteriophage nucleic acid. 2. Improved conditions were found and tested for blocking oligonucleotides with carbodi-imide and cleaving by ribonuclease A at cytidylate residues. 3. A synthetic medium is described which allows labelling in vivo with 32P to give specific radioactivities higher than those obtained in the procedures used previously.
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机译：1.确定了来自噬菌体R17的RNA基因组的73个核苷酸的序列。只能在一个翻译框架中通读。该片段不是外壳蛋白顺反子的一部分（Min Jou et al。，1972），也不来自先前描述的非翻译序列（Steitz，1969; Nichols，1970; Cory et al。，1970; de Wachter et al。）。 ，1971； Contreras等，1971； Cory等，1972）。它包含23个和24个核苷酸的两个序列，其中22个相同。这种重复是在噬菌体核酸中发现的第一个。 2.发现改善的条件，并测试了用碳二亚胺封闭寡核苷酸并通过核糖核酸酶A切割胞苷残基的条件。 3.描述了一种合成培养基，该培养基可以在体内用 32 P进行标记，从而获得比以前使用的方法更高的比放射性。
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