We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm−1–3400 cm−1), together with the commonly-measured C-H stretch (2750 cm−1–2970 cm−1) and SHG provide potentially important structural information and contrast in these materials.
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机译:我们展示了多峰相干反斯托克斯拉曼散射(CARS)显微镜的实用性,用于结构化缩合碳水化合物系统的研究。同时谐波产生(SHG)和光谱扫描CARS显微镜用于阐明纤维素棉纤维和经历快速热湿膨胀的淀粉颗粒的结构,排列和密度。我们的结果表明,OH拉伸区域(3000 cm -1 sup> –3400 cm -1 )的CARS响应,以及通常测量的CH拉伸(2750 cm > -1 sup> –2970 cm −1 sup>)和SHG在这些材料中提供潜在的重要结构信息和对比度。
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