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Potential Use of Rice Field Cyanobacterium Nostoc muscorum in the Evaluation of Butachlor Induced Toxicity and their Degradation

机译:稻瘟病菌粘液在稻田丁草胺诱导的毒性及其降解中的潜在应用

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摘要

In the present study, butachlor (5, 10, 20, 40 and 80 ppm) induced toxicity in Nostoc muscorum and their degradation was evaluated. The dose of butachlor dependent decreased in the cell survival and growth of N. muscorum was noticed. Scanning electron microscopy revealed the adverse impact on the cell size and shapes. Low concentrations of butachlor (10 and 20 ppm) induced the over expression of a polypeptides of 31.0 K Da and 42.7 K Da, respectively which could be responsible for developing resistance in the organism up to certain level. Further, the degradation product of butachlor as a result of metabolic activities of N. muscorum, identified by GC-MS analysis includes phenols and benzene dicarboxylic acid indicating the utilization of herbicide during active growth.
机译:在本研究中,丁草胺(5、10、20、40和80 ppm)在Nostoc muscorum中诱导了毒性,并对其降解进行了评估。注意到丁草胺依赖性剂量降低了粘液猪笼草的细胞存活和生长。扫描电子显微镜揭示了对细胞大小和形状的不利影响。低浓度的丁草胺(10和20 ppm)分别导致31.0 K Da和42.7 K Da的多肽过表达,这可能导致生物体产生一定程度的抗性。此外,通过GC-MS分析鉴定的由于粘菌猪笼草的代谢活性而导致的丁草胺的降解产物包括苯酚和苯二甲酸,表明在活跃生长期间利用了除草剂。

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