A butachlor-degrading bacterial strain named BTC-3 was isolated from a soil sample near the outfall of a pesticide factory, which had produced butachlor for many years.BTC-3 could degrade more than 85%of 100 mg/L butachlor in inorganic salt culture medium with butachlor as sole carbon source within 6 days.Combining the culture, physiological and biochemical character-istics with 16S rRNA sequence analysis, strain BTC-3 was identified as Bacillus sp.The optimal temperature and pH-value for the degradation of butachlor by BTC-3 were 30℃and 7, respectively.When the inoculation amount was less than or equal to 3%, the degrading rate increased with the increase in the inoculation amount.When the initial concentration of butachlor was less than or e-qual to 100 mg/L, the higher the concentration of butachlor, the better the effect of degradation.%从长期生产丁草胺的农药厂排水口土壤中分离得到1株能够降解丁草胺的细菌，将其命名为BTC－3。在以丁草胺为唯一碳源的基础盐培养基中，6 d内可将100 mg／L的丁草胺降解85％以上。经培养特征、生理生化分析和16S rRNA序列分析，将该菌株鉴定为芽孢杆菌属（ Bacillus sp．）。菌株BTC－3降解丁草胺的最适温度为30℃，最适pH值为7；当接种量≤3％时，接种量越大，降解率越高；当丁草胺初始浓度≤100 mg／L时，浓度越高，降解效果越好。
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