High level expression of Acidothermus cellulolyticus β-1 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid

摘要

BackgroundLarge-scale production of effective cellulose hydrolytic enzymes is the key to the bioconversion of agricultural residues to ethanol. The goal of this study was to develop a rice plant as a bioreactor for the large-scale production of cellulose hydrolytic enzymes via genetic transformation, and to simultaneously improve rice straw as an efficient biomass feedstock for conversion of cellulose to glucose.