Rapid high throughput template preparation (rHTTP) method: a novel cost effective method of direct PCR for a wide range of plants

摘要

BackgroundConventional plant DNA isolation methods are complex, time consuming and require technical expertise. These limitations were overcome using the DNA isolation kits which, however significantly add to the research costs. Hence the present study was aimed to develop a high throughput, rapid and inexpensive method of PCR ready DNA template preparation from plant materials.