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Deep sequencing reveals the complex and coordinated transcriptional regulation of genes related to grain quality in rice cultivars

机译:深度测序揭示了与水稻品种品质相关基因的复杂协调的转录调控

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摘要

BackgroundMilling yield and eating quality are two important grain quality traits in rice. To identify the genes involved in these two traits, we performed a deep transcriptional analysis of developing seeds using both massively parallel signature sequencing (MPSS) and sequencing-by-synthesis (SBS). Five MPSS and five SBS libraries were constructed from 6-day-old developing seeds of Cypress (high milling yield), LaGrue (low milling yield), Ilpumbyeo (high eating quality), YR15965 (low eating quality), and Nipponbare (control).
机译:背景碾米产量和食用品质是水稻的两个重要谷物品质特征。为了鉴定涉及这两个性状的基因,我们使用大规模平行签名测序(MPSS)和合成测序(SBS)对发育中的种子进行了深度转录分析。由6天龄的赛普拉斯(高碾米产量),LaGrue(低碾米产量),Ilpumbyeo(高食用质量),YR15965(低食用质量)和Nipponbare(对照)的发育中种子构建了五个MPSS和五个SBS库。

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