New pleiotropic effects of eliminating a rare tRNA from Streptomyces coelicolor revealed by combined proteomic and transcriptomic analysis of liquid cultures

摘要

BackgroundIn Streptomyces coelicolor, bldA encodes the only tRNA for a rare leucine codon, UUA. This tRNA is unnecessary for growth, but is required for some aspects of secondary metabolism and morphological development. We describe a transcriptomic and proteomic analysis of the effects of deleting bldA on cellular processes during submerged culture: conditions relevant to the industrial production of antibiotics.