Optimization of a large-scale gene disruption protocol in Dictyostelium and analysis of conserved genes of unknown function

摘要

BackgroundDevelopment of the post-genomic age in Dictyostelium will require the existence of rapid and reliable methods to disrupt genes that would allow the analysis of entire gene families and perhaps the possibility to undertake the complete knock-out analysis of all the protein-coding genes present in Dictyostelium genome.