In order to observe amino acid consumption during cultivation of Dictyoslelium discoideum on synthetic SIH medium, a method for determination of amino acids by reversed-phase high performance liquid chromatography(RP-HPLC) with 2, 4-dinitro-chlorobenzene derivatization was investigated. 16 amino acids could be well separated in 60 min,and a good linear relationship between peak area and concentration of amino acids was established with the correlation coefficients in the range of 0. 992-0. 999, Analysis results of samples of D. Discoideum on SIH medium indicated that lysine was completely consumed after 7 d of cultivation. Methionine, tryptophan.arginine and histidine were also utilized considerably while aspartic acid, glutamic acid, glycine, threonine, proline and valine did not show significant demand during the cell growth. This metabolic characteristic would supply reliable data to design a more reasonable synthetic medium for D. Discoideum.%为观察盘基网柄菌(Dictyostelium discoideum)在SIH培养基上对氧基酸的利用情况,以2.4-二硝基氯苯为衍生化试剂,研究了用反相高效液相色谱(RP-HPLC)测定氨基酸的方法.在60 min内16种氨基酸达到基线分离,峰面积与氨基酸浓度的线性相关系数为0.992～0.999.对发酵液样品的分析结果表明,盘基网柄菌对赖氨酸的利用最为彻底,发酵后期赖氨酸已被消耗完全.蛋氯酸、色氨酸,精氨酸、组氧酸也较易被盘基网柄菌利用,而对天冬氨酸、谷氨酸、甘氨酸、苏氨酸、脯氨酸、缬氨酸的需求不大.这一代谢特点为改进盘基网柄菌培养基组成提供依据.
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