Automated analysis of calcium spiking profiles with CaSA software: two case studies from root-microbe symbioses

摘要

BackgroundRepeated oscillations in intracellular calcium (Ca²⁺) concentration, known as Ca²⁺ spiking signals, have been described in plants for a limited number of cellular responses to biotic or abiotic stimuli and most notably the common symbiotic signaling pathway (CSSP) which mediates the recognition by their plant hosts of two endosymbiotic microbes, arbuscular mycorrhizal (AM) fungi and nitrogen fixing rhizobia. The detailed analysis of the complexity and variability of the Ca²⁺ spiking patterns which have been revealed in recent studies requires both extensive datasets and sophisticated statistical tools.