Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle sheep goat roe buck and deer by PCR-RFLP

摘要

BackgroundAs an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species.