BackgroundDue to the routine use of dexamethasone (DEX) in veterinary and human medicine and its negative impact on the rate of wound healing and skeletal muscle condition, we decided to investigate the effect of DEX on the inflammatory and repair phases of skeletal muscle regeneration. In this study, a porcine skeletal muscle injury model was used. The animals were divided into non-treated and DEX-treated (0.2 mg/kg/day) groups. On the 15th day of DEX administration, bupivacaine hydrochloride-induced muscle injury was performed, and the animals were sacrificed in subsequent days. Regeneration was assessed by histopathology and immunohistochemistry. In the inflammatory phase, the presence and degree of extravasation, necrosis and inflammation were evaluated, while in the repair phase, the numbers of muscle precursor cells (MPCs), myotubes and young myofibres were estimated.
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机译：背景由于地塞米松（DEX）在兽医和人类医学中的常规使用及其对伤口愈合速度和骨骼肌状况的负面影响，我们决定研究DEX对骨骼肌再生的炎症和修复阶段的影响。在这项研究中，使用了猪骨骼肌损伤模型。将动物分为未治疗组和DEX治疗组（0.2μg/ kg /天）。在DEX给药的第15天，进行盐酸布比卡因诱导的肌肉损伤，并在随后的几天处死动物。通过组织病理学和免疫组织化学评估再生。在炎性阶段，评估外渗，坏死和炎症的存在和程度，在修复阶段，评估肌肉前体细胞（MPC），肌管和年轻肌纤维的数量。
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