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Assessment of the main plasma parameters included in a metabolic profile of dairy cow based on Fourier Transform mid-infrared spectroscopy: preliminary results

机译:基于傅立叶变换中红外光谱法评估奶牛代谢谱中主要血浆参数:初步结果

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摘要

BackgroundAlthough a metabolic profile represents a valid tool utilized in dairy herds to determine abnormalities in blood chemistry related to an increased risk of production diseases, there are no studies on application of Fourier Transform mid-infrared (FT-MIR) spectroscopy. This study assesses the potential application of FT-MIR to analyze the main blood biochemical parameters included in the metabolic profile of dairy cows. Infrared transmission spectra were acquired for 35 plasma samples (two replicates on each sample) of Italian Friesian dairy cows (14 primiparous and 21 pluriparous), all without clinical events, and at different stages of lactation, although mainly in the transition phase. Each sample was also analyzed independently using accepted reference clinical chemical methods and these results were used as calibrating values to perform predictive models by PLS method using cross validation.
机译:背景技术尽管代谢谱代表着一种有效的工具,可用于确定奶牛群中与增加的生产疾病风险相关的血液化学异常,但尚无关于傅立叶变换中红外(FT-MIR)光谱技术应用的研究。这项研究评估了FT-MIR在分析奶牛代谢谱中主要血液生化参数方面的潜在应用。获得了意大利弗里斯兰奶牛(14头初产和21头多胎)的35个血浆样本(每个样本有两个重复)的红外透射光谱,尽管没有临床事件,但都处于泌乳的不同阶段,尽管主要在过渡阶段。还使用公认的参考临床化学方法对每个样品进行了独立分析,并将这些结果用作校准值,以使用交叉验证的PLS方法执行预测模型。

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