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Effect of abrupt weaning at housing on leukocyte distribution functional activity of neutrophils and acute phase protein response of beef calves

机译:断奶断奶对牛肉犊白细胞分布中性粒细胞功能活性和急性期蛋白反应的影响

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摘要

BackgroundSixteen, spring-born, single suckled, castrated male calves of Limousin × Holstein-Friesian and Simmental × Holstein-Friesian dams respectively, were used to investigate the effect of weaning on total leukocyte and differential counts, neutrophil functional activity, lymphocyte immunophenotypes, and acute phase protein response. Calves grazed with their dams until the end of the grazing season when they were housed in a slatted floor shed. On the day of housing, calves were assigned to a treatment, (i) abruptly weaned (W: n = 8) or (ii) non-weaned (controls) (C: n = 8). Weaned calves were housed in pens without their dams, whereas non-weaned (control) calves were housed with their dams. Blood was collected on day -7, 0 (housing), 2, 7, and 14 to determine total leukocyte and differential counts and concentration of fibrinogen and haptoglobin. Lymphocyte immunophenotypes were characterised using selected surface antigens (CD4+, CD8+, WC1+ (γδ T cells), MHC Class II+ lymphocytes), and the functional activities of neutrophils (surface expression of L-selectin (CD62L), phagocytic and oxidative burst activity) were investigated using flow cytometry.
机译:背景分别使用十六只出生于春季的利穆赞×荷斯坦-弗里斯兰大水牛和西门塔尔×荷斯坦-弗里斯兰大水牛的suck割雄性牛犊,研究了断奶对总白细胞和差异计数,中性粒细胞功能活性,淋巴细胞免疫表型和急性期蛋白反应。小牛与水坝一起吃草,直到放牧季节结束,他们被安置在板条地板棚中。在饲养当天,将犊牛指定为一种治疗方法,(i)断奶(W:n = 8)或(ii)不断奶(对照组)(C:n = 8)。断奶的犊牛被安置在没有坝的围栏中,而断奶的(对照)犊牛被安置在坝上。在第-7、0(居住),2、7和14天收集血液,以确定总白细胞和纤维蛋白原和触珠蛋白的差异计数和浓度。使用选定的表面抗原(CD4 + ,CD8 + ,WC1 + (γδT细胞),MHC II类)表征淋巴细胞的免疫表型使用流式细胞仪研究了+淋巴细胞和中性粒细胞的功能活性(L-选择蛋白的表面表达(CD62L),吞噬和氧化爆发活性)。

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